The development of real-time polymerase chain reaction using species-specific primer targeting cytochrome-b for identification of canine meat in mackerel meatballs for halal authentication

The practice of mixing food using canine meat has been widely found. This practice is motivated by the low price of canine meat in the market. The purpose of this research was to design a species-specific primer (SSP) and develop a real-time PCR method for analyzing canine meat (Canis lupus familiaris) in mackerel meatballs intended for halal authentication. The design of SSP was carried out in silico using NCBI-Primer BLAST and IDT sites. The primer specificity analysis was performed on DNA...