Comparison of the Conventional, Chemical, and Ultrasound Extraction of Crude Polysaccharides and Their Properties from Lentinula edodes (Berk.) Pegler

This study aimed to compare the efficiency of four extraction methods, hot water (HW), hot alkaline (HA), ultrasound-assisted water (UW), and ultrasound-assisted alkaline (UA), for extracting crude β-glucan from Lentinula edodes, focusing on yield, functionality, and antidi- abetic potential. The response surface methodology was used to optimize extraction condi- tions. Among all methods, UW yielded the highest β-glucan content (34.51 ± 0.82 g/100 g dry extract), indicating enhanced extraction...