This study aimed to compare the efficiency of four extraction methods, hot water (HW), hot alkaline (HA), ultrasound-assisted water (UW), and ultrasou

This study aimed to compare the efficiency of four extraction methods, hot water (HW), hot alkaline (HA), ultrasound-assisted water (UW), and ultrasound-assisted alkaline (UA), for extracting crude β-glucan from Lentinula edodes, focusing on yield, functionality, and antidi- abetic potential. The response surface methodology was used to optimize extraction condi- tions. Among all methods, UW yielded the highest β-glucan content (34.51 ± 0.82 g/100 g dry extract), indicating enhanced extraction...