Ultrasound-Assisted Extraction of Bioactives from Spirulina platensis: Optimization and Prediction of Their Properties Using Near-Infrared Spectroscopy Coupled with Artificial Neural Network Modeling

This study optimizes the ultrasound-assisted extraction (UAE) of bioactive compounds from Spirulina platensis and develops a rapid, non-destructive analytical method. A Box– Behnken design and desirability function were used to find the optimal extraction con- ditions to simultaneously maximize total polyphenols, proteins, C-phycocyanin, and an- tioxidant activity. The optimal conditions were a solid-to-liquid ratio of 35 g/L, a time of 20 min, a pH of 10, and a temperature of 45 ◦C....