Novel Environmentally-Friendly Process for Selective Extraction and Enrichment of DHAEPA-Containing Phospholipids from Krill Oil via Differential Temperature-Controlled Crystallization

This study presents a novel environmentally-friendly process for the selective extraction and enrichment of DHA/EPA-containing phospholipids (PL-DHA/EPA) from krill oil. The methodology leverages differential crystallization behavior between phospholipids and triacylglycerols in ethanolic solutions, exploiting their distinct freezing point thresholds to achieve precise fractionation. Response surface methodology optimization identified optimal extraction parameters: liquid-to-material ratio of...