Microsoft Word - foods-3932401 - aff changed - Copy.docx

The global rise in obesity and metabolic disorders has intensified the demand for safe and effective sugar alternatives. Monellin, a naturally sweet protein derived from Dioscore- ophyllum cumminsii, serves as an excellent sugar alternative, but its broader application has been constrained by poor thermal stability and limited evaluation of long-term meta- bolic effects. In this study, we evaluated the metabolic effects of MNEI-Mut6, a boiling- resistant single-chain monellin variant, in male...