Rapid Salmonella Serovar Classification Using AI-Enabled Hyperspectral Microscopy with Enhanced Data Preprocessing and Multimodal Fusion

Salmonella serovar identification typically requires multiple enrichment steps using selective media, consuming considerable time and resources. This study presents a rapid, culture- independent method leveraging artificial intelligence (AI) to classify Salmonella serovars from rich hyperspectral microscopy data. Five serovars (Enteritidis, Infantis, Kentucky, Johannesburg, 4,[5],12:i:-) were analyzed from samples prepared using only sterilized de-ionized water. Hyperspectral data cubes were...