The Functional Characterization of an AA10 Lytic Polysaccharide Monooxygenase from Saccharophagus degradans 2-40T for Enhanced Chitin Biodegradation

Lytic polysaccharide monooxygenases (LPMOs) represent copper-dependent enzymes pivotal in breaking down resilient polysaccharides like cellulose and chitin by means of oxidation, creating more accessible sites for glycoside hydrolases. To elevate the conversion efficiency of chitin, an AA10 LPMO was identified from the genome of Saccharophagus degradans 2-40T and heterologously expressed. The optimal pH for the activity of recombi- nant SdLPMO10A is 9.0, and the optimal temperature is 60 ◦C....